Table 2.
Transformation properties of E7 mutants with mutations in predicted hydrophobic core residues and in the zinc-coordinating cysteines, with a summary of dimerization potential in vivo
| Mutant | Mean (±SD) no. of colonies |
Transformation phenotype | Mean dimerization level (±SD) in vivo | |
|---|---|---|---|---|
| Mutant | Wild type | |||
| C58G/C91G | 4.46 ± 1.52 | 12.00 ± 3.00 | Decreased ability | 0.00 ± 0.00 |
| L65A | 12.00 ± 9.93 | 12.75 ± 2.87 | Transforms | 0.03 ± 0.00 |
| L67R | 8.00 ± 3.36 | 16.25 ± 3.40 | Decreased ability | 0.44 ± 0.02 |
| V69A | 7.33 ± 3.51 | 14.16 ± 5.29 | Decreased ability | 0.05 ± 0.01 |
| I76A | 13.00 ± 6.97 | 12.00 ± 3.46 | Transforms | 0.28 ± 0.02 |
| L79A | 4.00 ± 3.60 | 12.00 ± 3.00 | Decreased ability | 0.23 ± 0.02 |
| LLMG82-85AAAD | 7.50 ± 7.45 | 11.25 ± 2.87 | Transforms | 0.71 ± 0.12 |
| L87A | 11.00 ± 7.87 | 10.25 ± 4.27 | Transforms | 0.01 ± 0.00 |
| I89A/V90A | 10.50 ± 6.19 | 10.25 ± 4.27 | Transforms | 0.03 ± 0.01 |
| C91G | 5.25 ± 4.27 | 13.75 ± 4.27 | Decreased ability | 0.00 ± 0.00 |