Fig. 2.

Kinetics of viral replication of rgDkYK10 and rgDkYK10-B5 in tissues from chickens and virus shedding in oral and cloacal swabs. Chickens were inoculated intranasally with 10⁶ EID₅₀ of rgDkYK10 or rgDkYK10-B5 (n = 3 for each inoculation group). Lungs (A), spleens (B), and brains (C), as well as oral (D) and cloacal (E) swabs, were collected sequentially at 24, 48, 60, and 84 h p.i., and their virus titers were determined with embryonated chicken eggs. The mean virus titers ± standard deviations from three birds at each point are shown (log₁₀ EID₅₀/g). *, significant difference (P < 0.05).