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Immunohistochemical detection of AIV matrix protein and TUNEL staining of apoptotic cells in tissues from chickens infected with rgDkYK10 or rgDkYK10-B5. AIV M protein was detected in the lungs (A and E), spleens (B and F), and brains (C and G) from chickens intranasally inoculated with rgDkYK10 (A to C) or rgDkYK10-B5 (E to G) at 48 h p.i. The apoptotic cells were detected in the lung tissue of chickens infected with rgDkYK10 (D) or rgDkYK10-B5 (H) at 48 h p.i. Scale bars = 50 μm.
