Fig. 2.

Restriction analysis of geographically diverse M. fascicularis macaques. NsiI restriction analysis was used to determine the splice site genotype. The uncut band, which is associated with the G allele and the absence of TRIMCyp, is expected to be approximately 1,194 bp; the cut band associated with the T allele and the presence of TRIMCyp is expected to be approximately 891 bp. The inferred genotype is shown below the gel. (A) Animals of Indonesian origin housed at the WaNPRC. (B) Samples from Indonesia, Indochina, Mauritius, and the Philippines. Samples tested in panel A were used as controls (labeled) to test for the completion of digestion in independent assays. For inferring the genotype, the band density was quantified by using Quantity One software (Bio-Rad) and compared to those of controls processed at the same time.