Fig. 4.

Phylogenetic tree of the intron 6-exon 8 region. The intron 6-exon 8 region of the TRIM5 gene was cloned and sequenced. M. fascicularis animals are classified based on geographic origin; other species are as indicated. All WaNPRC M. fascicularis animals are of Indonesian origin. Multiple clones were sequenced from each WaNPRC animal; thus, heterozygotes are present in two locations on the tree. Only one clone was sequenced from each of the other individuals. Sequences from Papio, M. thibetana, M. nigra, M. sylvanus, and six of the WaNPRC M. fascicularis animals were described previously (5). The tree was built using a maximum parsimony algorithm (33), using the human sequence as an outgroup. Similar results were also found by use of maximum likelihood and neighbor-joining algorithms (not shown). Bootstrap values from 1,000 replicates are shown.