Fig. 7.

Amino acid 178 affects TRIMCyp protein expression or stability. (A) Anti-HA immunoblot of control and mutagenized TRIMCyp-expressing CrFK cell lines. Cell lines expressing wild-type and H178Y TRIMCyp from animals M05221 and M05386 were transduced and grown in parallel. (B) Restriction activity against HIV-1 NL4-3. (C) Restriction activity against HIV-2 ROD. (D) Restriction activity against FIV. Genotypes at amino acid positions 369 and 446 are shown, as described in the legend of Fig. 5. Data shown are means ± standard deviations from three independent experiments using one representative dilution of virus (11 ng RT/well for HIV-1 and HIV-2 and 33 ng RT/well for FIV). Cell lines expressing wild-type TRIMCyp from animals M05221 and M05386 had no or negligible restriction activity (not shown).