Fig. 2.

K254 of the P protein is one sumoylation site. (A) Sumoylation of P-K254R. K254 was mutated to arginine (R), and this P-K254R was used for IP-IB to compare the sumoylation level to that of wild-type P. (B) Quantification of the sumoylation level. Data from three individual experiments from panel A were used for quantification and statistical analysis. The ratio of the singly sumoylated P protein to total P protein was set to 1 for wild-type P protein, and the relative sumoylation level of P-K254R was normalized to that of wild-type P. The P value was calculated using a t test.