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P-K254R has lower activity in PIV5 minigenomes. (A) Minigenome activity of P-K254R. Increasing amounts of P-K24R or the P protein were used in the minigenome system. Immunoblotting was performed to show the input amounts of NP, P, or P-K254R. (B) CD analysis of His-P-WT and His-P-K254R purified from bacteria.
