Fig. 8.

Interactions of P with NP, L, and itself. (A) Interaction between NP and P-WT/P-K254R. BSR-T7 cells were transfected with NP, along with P-WT or P-K254R, and metabolically labeled with [³⁵S]Met-Cys. Lysates of the labeled cells were used for immunoprecipitation with anti-P antibody or anti-NP antibody. (B) Interaction between L and P-WT/P-K254R. BSR-T7 cells were transfected with Flag-L along with P-WT or P-K254R. The transfected cells were lysed and used for IP with anti-P antibody and IB using anti-Flag antibody. Aliquots of the cells lysates were used for immunoblotting to show the input amounts of L and P-WT/P-K254R. (C) Tetramer formation of P-WT/P-K254R. The transfected cells were labeled with [³⁵S]Met-Cys, and DSP was used for cross-linking followed by immunoprecipitation using anti-P antibody. Half of the IP products were mixed with SDS loading buffer without DTT, and the other half were mixed with SDS loading buffer with DTT.