Fig. 2.
Vpu-deficient SHIVs produce less cell-free virus in rhesus PBMCs than wt SHIVs. (A) CD4+ T cells were isolated from PBMCs of four rhesus macaques and processed for FACS analysis as described in Materials and Methods. Black lines, BST-2 staining; gray lines, isotype controls. (B) ConA-activated rhesus PBMCs were infected with equal reverse transcriptase units of SHIVDH12-CL7 variants (top) or SHIVAD8-MV variants (bottom). All virus stocks were produced in transfected 293T cells. Virus replication was monitored by measuring the virus-associated RT activity in the culture supernatants over time.