Inhibition of HSV-1 mRNA and DNA. (A) HSV-1 UL54, UL30, and UL27 gene expression levels were measured by real-time PCR and normalized to GAPDH levels. Nucleic acids were extracted from HSV-1-infected wild-type A1-expressing and control RSCs at 8 hpi. (B) DNA copy number was measured by real-time PCR in A1-expressing and control RSCs 4 and 8 hpi. As a control, UV-inactivated virus was also used to infect control RSCs. **, P < 0.05; ***, P < 0.005.