Fig. 3.
Infectivity of EIAV variants PND-1 (black bars) and PND-5 (gray bars). (A) Cell-free infectivity of EIAVPND-1 and EIAVPND-5 stocks. The RNA copy number and infectious titer (focus-forming units/ml) represent the virus effects ± 1 standard deviation, as estimated from a linear mixed-effect model. (B) Single-cycle infectivity of PND-1- and PND-5-pseudotyped viruses carrying the firefly or Renilla luciferase reporter gene. ED cells were coinfected with equal RNA copy numbers of the viruses, and infectivity was calculated as a ratio, the number of infected cells per RNA copy number. (C) Cell-associated infectivity of EIAVPND-1 and EIAVPND-5, as determined by infectious center assay. Infectivity is calculated as a ratio, the number of infected target cells per donor cell. Two independent cell clones for each virus were assayed in triplicate, and the estimates represent the virus effects ± 1 standard deviation in a linear mixed-effect model, where the cell clone is a random effect.