Table 1.

Primer and probe sequences used in this study

Target	Primer or probea	Sequence (5′–3′)b
EIAV Env	Common forward	CGG GGT GTA GAC CAT TTC AA
	Common reverse	TGA CAA TGA TCA CTA TCT GTG CAA
	Common probe	HEX-TTA TGA GAC CAA TAG AAG CAT GCA-3IALK_FQ
	PND-1 reverse	TCC GTA CTC TCA TTT ACA GGG T
	PND-5 reverse	CAG TAA GGG TCG GAG GAA CCT G
EIAV Gag	Gag forward	AGC CAG GAC ATT TAT CTA GTC AAT GTA GAG CAC C
	Gag reverse	GTG CTG ACT CTT CTG TTG TAT CGG GAA AGT TTG
	Gag probe	56-FAM-ACG GGA AGC AAG GGG CTC AAG GGA GGC C-3IALK_FQ
Luciferase	Firefly luc forward	TAT GAA GAG ATA CGC CCT GGT T
	Firefly luc reverse	GCC CAT ATC GTT TCA TAG CTT C
	Renilla luc forward	GCA ATA GTT CAC GCT GAA AGT G
	Renilla luc reverse	CAA CAT GGT TTC CAC GAA GAA GT
Pseudovirus RNA	WHV PRE forward	CTG TTC CTG TTA ATC AAC CTC TGG
	WHV PRE reverse	ATA CAA GGA GGA GAA AAC GAA AGC
Equine β2M	E-β2M forward	TCT TTC AGC AAG GAC TGG TCT TT
	E-β2M reverse	CTA CCA CAC CAT TGG GAG TAA A
	E-β2M probe	56-ROXN-ATC TTC TGG TCC ATA CTG A-3IAbRQSp

^aluc, luciferase; E-β2M, equine β2 microglobulin.

^bHEX, hexachlorofluorescein; 3IALK_FQ, Iowa Black FQ quencher (IDT); 56-FAM, 5,6-carboxyfluorescein; 56-ROXN, carboxy-X-rhodamine; 3IAbRQSp, Iowa Black FQ quencher (IDT).