Fig. 2.
(A) Single and double TGMV AL1 mutant interactions with NbSCE1. Interactions were assayed by measuring the β-galactosidase activity in total protein extracts and are expressed as a percentage of the interaction between the wild-type (Wt) AL1 protein and NbSCE1. The negative control corresponds to yeast coexpressing S. cerevisiae ULP1 (ubiquitin-like protein) and NbSCE1. The bars correspond to data from an average of three independent experiments, each assayed for four independent transformants. The error bars correspond to two standard errors. (B) Immunoblot analysis of protein extracts from yeast cells coexpressing NbSCE1 and AL1 using an anti-AL1 polyclonal antibody. The molecular mass for the observed band is 58 kDa.