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. 2011 Oct 18;5(10):e1338. doi: 10.1371/journal.pntd.0001338

Figure 2. Determination the 5′ end of GlsR2-derived small RNA by primer extension.

Figure 2

A 17 nt end-labeled primer complementary to the 3′ end of GlsR2 were used in the primer extension reactions. In vitro transcribed GlsR2 (0.75 ng) was used as a control template (Con) to show the secondary structure stops. Size-fractioned small RNA (<200 nts, 4 µg) was used as another template (RNA) to identify the mature miR5 and its potential intermediary precursor. GlsR2 sequencing reactions, using the same primer, were run along with the primer extension to determine the sequences of the products.