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. 2011 Oct 18;5(10):e1338. doi: 10.1371/journal.pntd.0001338

Figure 5. miR5 was co-immunoprecipitated with GlAgo.

Figure 5

The ∼26 nt RNA band co-immunoprecipitated with 3×HA GlAgo [21] was analyzed by RT-qPCR using TaqMan probe specific for miR5. GlAgo: RNA template extracted from the immunoprecipitate of 3×HA GlAgo expressing cells. WB: RNA template extracted from the immunoprecipitate of control cells. The qPCR products were run on a 2% agarose gel. The specific band at ∼70 bp (26 nts plus the linker) showed miR5 co-immunoprecipitated with 3×HA GlAgo (upper panel). The Ct values between the two samples differed by about 9 cycles, suggesting that miR5 bound specifically to 3×HA GlAgo and became enriched in the immonoprecipitate (lower panel).