Table 1. Real-time PCR assay panel and results used to profile case isolate.

Genomic target	Result	Specificity	Target info
TTS1	Pos.	B.ps.	Type III secretion gene cluster [6]
YLF	Pos.	SEA isolates of B.ps.	Yersinia-like fimbrial gene cluster; mutually exclusive of BTFC; mostly found in B.ps. from Thailand [7]
BTFC	Neg.	Australian isolates of B.ps.	B. thailandensis-like flagella & chemotaxis gene cluster; mutually exclusive of YLF; mostly found in B. ps. from Australia [7]
cheB	Neg.	B. thailandensis	B. thailandensis homolog of BTFC [7]
wcbG	Pos.	B.ps. virulence gene	A gene in the wcb capsule biosynthesis operon; more prevalent in clinical isolates than environmental isolates of B. ps. (A. Tuanyok, unpublished data)
fhaB_1	Pos.	B.ps. virulence gene	Filamentous hemagglutinin (adhesin; a virulence molecule produced by intracellular bacteria). B.ps. fhaB genes vary in sequence and copy number among strains and may infer geographic origin. [8]
fhaB_2	Pos.	B.ps. virulence gene
fhaB_3	Pos.	B.ps. virulence gene
bimA Bm	Neg.	Most B.m., some B.ps.	Burkholderia intracellular motility A protein (a virulence factor) – B. mallei dominant [9]
bimA Bp	Neg.	Most B.ps.	Burkholderia intracellular motility A protein (a virulence factor) – B. pseudomallei dominant [9]
bpaA	Neg.	B.ps. virulence gene	Virulence gene located on a genomic island [8]
BPSS0654	Pos.	B.ps. metabolic gene	Common metabolic gene on genomic islands [8]
BurkDiff	B.ps. Pos.B.m. Neg.	B.m. and B.ps.	Single nucleotide polymorphism differentiating B.m. from B.ps. Flanking region is unique to these two species. (10)

(B.ps. = B. pseudomallei; B.m. = B. mallei; Pos. = Positive; Neg. = Negative; SEA = Southeast Asia).