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. 2011 Oct 18;6(10):e25589. doi: 10.1371/journal.pone.0025589

Figure 3. Western Blot analysis of UPR-related proteins.

Figure 3

Immunoblotting was performed on A. colonic and B. ileal samples of 5 healthy controls (HC), 5 active ulcerative colitis (UC) and 5 active Crohn's disease (CD) patients. Signals of HSPA5 (Fig. 3C), PDIA4 (Fig. 3D), XBP1s (Fig. 3E), GADD34 (Fig. 3F), and pEIF2A/EIF2A (Fig. 3G), were quantified. In colonic samples, significant increased concentrations of PDIA4 and pEIF2A/EIF2A were observed in both UC and CD patients, while a significant increase in HSPA5 was only found in UC patients. In ileal samples, no differential expression of the UPR-related proteins was observed. Levels in healthy controls were arbitrary set as 1, and UC and CD levels expressed as the ratio to healthy controls (*P<0.05, **P<0.01).