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. 2011 Oct 18;6(10):e25589. doi: 10.1371/journal.pone.0025589

Table 3. Sequences of qRT-PCR primers, amplicon lengths (bp), PCR efficiencies (%) and correlation coefficients(R2).

Gene symbol Accession number Primer sequence (Forward and Reverse) Amplicon (bp) Efficiency (%) R2
GAPDH NM_002046 F_TGCACCACCAACTGCTTAGC 87 91 0.9936
R_GGCATGGACTGTGGTCATGAG
SDHA NM_004168 F_TGGGAACAAGAGGGCATCTG 86 98 0.9947
R_CCACCACTGCATCAAATTCATG
HPRT NM_000194 F_TGACACTGGCAAAACAATGCA 94 92 0.9985
R_GGTCCTTTTCACCAGCAAGCT
IL8 NM_000584 F_GAATGGGTTTGCTAGAATGTGATA 129 99 0.9995
R_CAGACTAGGGTTGCCAGATTTAAC
HSPA5, BiP, GRP78 NM_005347 F_GGGAACGTCTGATTGGCGAT 69 106 0.9996
R_CGTCAAAGACCGTGTTCTCG
XBP1_U NM_005080 F_AGACAGCGCTTGGGGATGGAT 133 98 0.997
R_CCTGCTGCAGAGGTGCACGTAG
XBP1_S NM_001079539 F_AGACAGCGCTTGGGGATGGAT 107 103 0.9971
R_CCTGCACCTGCTGCGGACTC
PDIA4, ERp72 NM_004911 F_TCCCATTCCTGTTGCCAAGAT 121 99 0.9923
R_GCCCTCGTAGTCTACAGCCT
HMOX1 NM_002133 F_CAGTGCCACCAAGTTCAAGC 112 102 0.9867
R_GTTGAGCAGGAACGCAGTCTT
PPP1R15A, GADD34 NM_014330 F_TCCTCTGGCAATCCCCCATA 112 109 0.9984
R_GGAACTGCTGGTTTTCAGCC
DDIT3, CHOP NM_001195053 F_AAGGCACTGAGCGTATCATGT 105 102 0.9913
R_TGAAGATACACTTCCTTCTTGAACA
EIF2A NM_032025 F_CTGCACTCCTTCGATCTTCTG 68 105 0.9906
R_AGTTGTAGGTTGGGTATCCCAG
ATF6 NM_007348 F_TCAGACAGTACCAACGCTTATGC 113 97 0.9952
R_GTTGTACCACAGTAGGCTGAGA
ERO1L NM_014584 F_GCCAGGTTAGTGGTTACTTGG 141 108 0.9938
R_GGCCTCTTCAGGTTTACCTTGT
ERN1,IRE1 NM_001433 F_TTTGGAAGTACCAGCACAGTG 184 100 0.9911
R_TGCCATCATTAGGATCTGGGA
ATF4 NM_001675 F_GACCACGTTGGATGACACTTG 154 97 0.9976
R_GGGAAGAGGTTGTAAGAAGGTG
NQO1 NM_000903 F_GGCAGAAGAGCACTGATCGTA 145 96 0.9881
R_TGATGGGATTGAAGTTCATGGC
EIF2AK3, PERK NM_004836 F_TGCCTGGCTCGAAGCACCAC 112 101 0.97
R_TGGTGCATCCATTGGGCTAGGA
DNAJC3, P58IPK NM_006260 F_TTTGCGTTCACAAGCACTTAAC 101 94 0.97
R_GTTCTGCATCCCAAACACAAAC
DNAJB9, ERDJ4 NM_012328 F_GGTGTGCCAAAATCGGCATC 185 100 0.98
R_GCACTGTGTCCAAGTGTATCATA