Table 3. Sequences of qRT-PCR primers, amplicon lengths (bp), PCR efficiencies (%) and correlation coefficients(R2).
Gene symbol | Accession number | Primer sequence (Forward and Reverse) | Amplicon (bp) | Efficiency (%) | R2 |
GAPDH | NM_002046 | F_TGCACCACCAACTGCTTAGC | 87 | 91 | 0.9936 |
R_GGCATGGACTGTGGTCATGAG | |||||
SDHA | NM_004168 | F_TGGGAACAAGAGGGCATCTG | 86 | 98 | 0.9947 |
R_CCACCACTGCATCAAATTCATG | |||||
HPRT | NM_000194 | F_TGACACTGGCAAAACAATGCA | 94 | 92 | 0.9985 |
R_GGTCCTTTTCACCAGCAAGCT | |||||
IL8 | NM_000584 | F_GAATGGGTTTGCTAGAATGTGATA | 129 | 99 | 0.9995 |
R_CAGACTAGGGTTGCCAGATTTAAC | |||||
HSPA5, BiP, GRP78 | NM_005347 | F_GGGAACGTCTGATTGGCGAT | 69 | 106 | 0.9996 |
R_CGTCAAAGACCGTGTTCTCG | |||||
XBP1_U | NM_005080 | F_AGACAGCGCTTGGGGATGGAT | 133 | 98 | 0.997 |
R_CCTGCTGCAGAGGTGCACGTAG | |||||
XBP1_S | NM_001079539 | F_AGACAGCGCTTGGGGATGGAT | 107 | 103 | 0.9971 |
R_CCTGCACCTGCTGCGGACTC | |||||
PDIA4, ERp72 | NM_004911 | F_TCCCATTCCTGTTGCCAAGAT | 121 | 99 | 0.9923 |
R_GCCCTCGTAGTCTACAGCCT | |||||
HMOX1 | NM_002133 | F_CAGTGCCACCAAGTTCAAGC | 112 | 102 | 0.9867 |
R_GTTGAGCAGGAACGCAGTCTT | |||||
PPP1R15A, GADD34 | NM_014330 | F_TCCTCTGGCAATCCCCCATA | 112 | 109 | 0.9984 |
R_GGAACTGCTGGTTTTCAGCC | |||||
DDIT3, CHOP | NM_001195053 | F_AAGGCACTGAGCGTATCATGT | 105 | 102 | 0.9913 |
R_TGAAGATACACTTCCTTCTTGAACA | |||||
EIF2A | NM_032025 | F_CTGCACTCCTTCGATCTTCTG | 68 | 105 | 0.9906 |
R_AGTTGTAGGTTGGGTATCCCAG | |||||
ATF6 | NM_007348 | F_TCAGACAGTACCAACGCTTATGC | 113 | 97 | 0.9952 |
R_GTTGTACCACAGTAGGCTGAGA | |||||
ERO1L | NM_014584 | F_GCCAGGTTAGTGGTTACTTGG | 141 | 108 | 0.9938 |
R_GGCCTCTTCAGGTTTACCTTGT | |||||
ERN1,IRE1 | NM_001433 | F_TTTGGAAGTACCAGCACAGTG | 184 | 100 | 0.9911 |
R_TGCCATCATTAGGATCTGGGA | |||||
ATF4 | NM_001675 | F_GACCACGTTGGATGACACTTG | 154 | 97 | 0.9976 |
R_GGGAAGAGGTTGTAAGAAGGTG | |||||
NQO1 | NM_000903 | F_GGCAGAAGAGCACTGATCGTA | 145 | 96 | 0.9881 |
R_TGATGGGATTGAAGTTCATGGC | |||||
EIF2AK3, PERK | NM_004836 | F_TGCCTGGCTCGAAGCACCAC | 112 | 101 | 0.97 |
R_TGGTGCATCCATTGGGCTAGGA | |||||
DNAJC3, P58IPK | NM_006260 | F_TTTGCGTTCACAAGCACTTAAC | 101 | 94 | 0.97 |
R_GTTCTGCATCCCAAACACAAAC | |||||
DNAJB9, ERDJ4 | NM_012328 | F_GGTGTGCCAAAATCGGCATC | 185 | 100 | 0.98 |
R_GCACTGTGTCCAAGTGTATCATA |