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. 2011 Oct 18;6(10):e25578. doi: 10.1371/journal.pone.0025578

Figure 1. Agrobacterium cell growth, vir gene expression, attachment to host cells, and host defense response in the presence of exogenous VirB5.

Figure 1

(A) SDS PAGE analysis of purified VirB5. (B) Growth curve of Agrobacterium. Gray squares, no VirB5; black squares, +VirB5. (C) vir gene induction by acetosyringone. MU, Miller units. (D) Agrobacterium attachment to plant tissues. CFU, colony-forming units. All data represent average values of three independent experiments with indicated standard deviations. Results obtained in VirB5-treated samples were not statistically different from untreated controls (P-values>0.2). (E) RT-PCR analysis of induction of PR1 gene expression by treatment with salicylic acid. Lane 1, control; lane 2, +salicylic acid; lane 3, +VirB5; lane 4, +VirB5+salicylic acid. (E) RT-PCR analysis of induction of 4-CL1 gene expression by treatment with flagellin 22. Lane 1, control; lane 2, +flagellin 22; lane 3, +VirB5; lane 4, +VirB5+flagellin 22. Constitutively expressed ACTIN (ACT) was used as internal control (lower panels).