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. 2011 Oct 18;6(10):e25578. doi: 10.1371/journal.pone.0025578

Figure 4. Effect of VirB5 secreted from transgenic plants on T-DNA expression.

Figure 4

(A) Subcellular localization of GFP-secVirB5 in tobacco leaf cells. (B) Subcellular localization of GFP-secVirB5 in plasmolysed tobacco leaf cells. GFP is in green, plastid autofluorescence is in red. All images are single confocal sections. (C) RT-PCR analysis of secvirB5 gene expression in low and high expresser transgenic lines. Lane 1, wild-type plant; lane 2, SB5-2; lane 3, SB5-3. Constitutively expressed ACTIN (ACT) was used as internal control (lower panel). (D) T-DNA expression in inoculated leaf discs from the SB5-2 and SB5-3 lines that express secVirB5, and from the B5 line that expresses VirB5. Control expression level in the wild-type plants (WT, black bar) was set at 100%. All data represent average values of three independent experiments with indicated standard deviations. Asterisk indicates a statistical significance (P-value<0.05) of the difference between the experimental and control data.