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. 2011 Oct 18;6(10):e25580. doi: 10.1371/journal.pone.0025580

Figure 1. The effect of PKC inhibitors on CSF-1 dependent mitogenesis.

Figure 1

(A) PKC immunoblots. “+” refers to the presence of a competing peptide. Arrow points to the PKC band that is competed off by the corresponding peptide. The lower band in the PKCζ blot is the authentic PKCζ protein (see text). (B) MTS assay. 32D.R cells were plated under the conditions shown and in the presence of 5 nM CSF-1. MTS activity was assayed after 48 h. (C) MTS assay was performed as described in (A) in the presence of the indicated amounts of PKCζ pseudosubstrate peptide. (D) MTS assay in the presence of CSF-1 and the indicated inhibitors, 10 µM U0126 and 5 µM LY294002.