Skip to main content
. 2011 Oct 18;6(10):e25368. doi: 10.1371/journal.pone.0025368

Figure 5. Schematic representation of selected yeast 2-hybrid clones and results.

Figure 5

TP, transit peptide; Roman numerals, transmembrane domains; CR, conserved region; PDV2, PDV2 binding domain. A, CJD1 protein. The amino terminal soluble portion (amino acids 60–164) was used as the bait. This peptide includes the J-like domain (amino acids 74–135). B, ARC6 clones and Y2H results. Top drawing: modular organization of ARC6. Second drawing: representative clone identified by library screening. This clone has the full J-like domain and the shortest CR domain of the six clones recovered (see Table 2 for details of other clones). Constructs defined by bracket: results from directed Y2H screening of truncated ARC6 proteins with positive and negative results indicated with ‘+’ and ‘−’, respectively.