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. 2011 Oct 18;6(10):e26142. doi: 10.1371/journal.pone.0026142

Figure 1. ICOVIR5-TK-L.

Figure 1

(A) Schematic representation of adenoviral genomic elements. The E1A-Δ24 gene was cloned under the control of the E2F-1 promoter, preceded by an insulator sequence in the 5′ region. The Tat8TK transgene was cloned downstream of L5 to be expressed as a part of a polycystronic transcript, under the control of the Major Late Promoter. (B) Quality control assessment. The presence of E2F-1 promoter, and of Hexon, E1A and Tat8TK genes was analyzed by RT-PCR in pancreatic cancer cells transduced with 1000 vp/cell of ICOVIR5-TK-L. A non-retrotranscribed mRNA sample was used as a negative control for the PCR.