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. 2011 Oct 18;6(10):e26135. doi: 10.1371/journal.pone.0026135

Figure 2. GGTI P61-E7 is a more potent inhibitor of geranylgeranylation compared with P61-A6.

Figure 2

(A and B), P61-E7 or P61-A6 treatment inhibits Rap1 geranylgeranylation in Panc-1 (A) and NIH3T3 (B) cells. Whole cell lysates from cells treated with DMSO, P61-A6, or P61-E7 for 48 h were prepared and resolved on SDS-PAGE for immunoblotting analysis using antibodies against the unprenylated form of Rap1 (U-Rap1, upper panels, A and B), or HDJ2 (loading control, lower panels, A and B). Data shown are representative of three independent experiments. The U-Rap1 and HDJ2 bands were quantified using ImageJ. Intensities of U-Rap1 bands were normalized to their respective loading control, and the results are given above the images as fold change compared to the DMSO control. (C) Whole cell lysates from NIH3T3 cells treated with DMSO, P61-A6, P61-E7, or FTI BMS-225975 for 48 h were prepared and resolved on SDS-PAGE for immunoblotting analysis using antibodies against the unprenylated form of Rap1 (U-Rap1, upper panel), or HDJ2 (lower panel). Data shown are representative of three independent experiments.