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. 2011 Oct 18;6(10):e26135. doi: 10.1371/journal.pone.0026135

Figure 7. Stable expression of farnesylated RhoA promotes partial resistance to the anti-proliferative effect of P61-E7.

Figure 7

(A) Stable expression of farnesylated RhoA (3xHA-RhoA-F) partially prevents P61-E7 inhibition of proliferation in Panc-1 cells in a manner that depends on the expression levels of 3xHA-RhoA-F. Panc-1 cells and Panc-1 stable cell lines expressing either wild-type 3xHA- RhoA-GG (clone no. 9) or RhoA-F mutant (clone no. 1, 4, or 8) were treated with DMSO or various concentrations of P61-E7 for 72 h. Cell number was determined as described in “Materials and Methods.” Cell proliferation relative to the DMSO control (100% value) is plotted. Data shown are averages ± S.D. of three independent experiments. (B) Protein levels of 3xHA-RhoA-GG (clone no. 9) and 3xHA-RhoA-F (clone no. 1, 4, or 8) in different clones of Panc-1 stable cell lines as analyzed by immunoblotting using antibodies against the HA tag or actin (loading control).