Skip to main content
. 2011 Oct 18;6(10):e26135. doi: 10.1371/journal.pone.0026135

Figure 8. Stable expression of farnesyalted RhoA rescues cells from cell rounding and inhibition of focal adhesion assembly caused by P61-E7.

Figure 8

Panc-1 cells, Panc-1 cells stably expressing 3xHA-RhoA-GG wild-type, and Panc-1 cells stably expressing 3xHA-RhoA-F mutant were serum-starved in the presence of DMSO or 5 µM P61-E7 for 48 h, followed by stimulation with 10% FBS in DMEM. Actin fibers and focal adhesions were visualized with FITC-phalloidin and mouse anti-vinculin followed by TRITC-conjugated anti-mouse antibody, respectively. Data are representative of three independent experiments.