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. 2011 Oct 18;6(10):e25794. doi: 10.1371/journal.pone.0025794

Figure 4. Relative Luciferase Activity of Constructs with HLA-DRB1 Derived Promoter and 5′-UTR Sequences.

Figure 4

A: luciferase activity was tested for the plasmids into which the different DRB1 lineage derived promoter sequences with lineage 2 derived 5′-UTR were inserted. B: Luciferase activity was tested for the plasmids into which the promoter sequences from DRB1 lineage 1 with different lineage-derived 5′-UTR were inserted. C: Luciferase activity was tested for the plasmids into which the different DRB1 lineage-derived promoter sequence with or without its 5′-UTR were inserted. Firefly luciferase activity of each sample was normalized by Renilla luciferase activities. The P4-5′U4 (A), P1-5′U4 (B) and pGL3.0 control vectors (C) were used as controls, and the normalized luciferase activities of controls were set as relative activity 1. Therefore no error bars were shown for the P4-5′U4, P1-5′U4 and pGL3.0 control vector. The relative luciferase activity of the other samples was calculated by their normalized luciferase activity to that of the normalized controls. Error bars represent standard error of the mean estimated from three separate experiments. *, P<0.05; ***, P<0.001, compared with the control or comparison between two groups as indicated.