Skip to main content
. 2011 Oct 18;6(10):e26285. doi: 10.1371/journal.pone.0026285

Table 2. Primers used for RT-PCR and qPCR analysis.

Target Gene Primers Sequence TA(°C) Cycles
RUNX-2 sense 5′-ACGACAACCGCACCATGGT-3′antisense 5′-CTGTAATCTGACTCTGTCCT-3′ 59 30
alkaline phosphatase (ALP) sense 5′-TGGAGCTTCAGA AGCTCAACACCA-3′antisense 5′-ATCTCGTTGTCTGAGTACCAGTCC-3′ 59 28
osteocalcin (OC) sense 5′-ATGAGAGCCCTCACACTCCTC-3′antisense 5′-GCCGTAGAAGCGCCGATAGGC-3′ 59 30
peroxisome proliferators-activated receptor 2 (PPAR-2) sense 5′-CGAGGGCGATCTTGACAGGAA -3′antisense 5′-CAGGGGGGTGATGTGTTTGAAC- 3′ 55 30
adipogenic protein (APP) sense 5′-CTGTCCAAGTCCAACAGCAA-3′antisense 5′-ACGTTGGCAGCTTTACGTCT-3′ 55 30
lipoprotein lipase (LPL) sense 5′-TCCGCGTGATTGCAGAGAGAG-3′antisense 5′-TGCTGCTTCTTTTGGCTCTGACT-3′ 55 30
aggrecan (Agg) sense 5′-TGAGGAGGGCTGGAACAAGTACC-3′antisense 5′-GGAGGTGGTAATTGCAGGGAACA-3′ 57 28
collagen II (Col II) sense 5′-TTTCCCAGGTCAAGATGGTC-3′antisense 5′-TCACCTGGTTTTCCACCTTC-3′ 57 30
SOX-9 sense 5′-TGGCCGAGATGATCCTAAAAATAA -3′antisense 5′-GCGCTTGGATAGGTCATGTTTGT-3′ 57 30
SOX-2 sense 5′-CCCCTGTGGTTACCTCTTCCTC-3′antisense 5′-GGCCGCTCTGGTAGTGCTG-3′ 61 32
NANOG sense 5′-ACCCCGTTTCACTGTGTTAGC-3′antisense 5′-GACGGCAGCCAAGGTTATTAAA-3′ 63 32
OCT4 sense 5′-GGCAAGCGATCAAGCAGCGAC-3′antisense 5′-GGGAAAGGGACCGAGGAGTAC-3′ 61 32
β-actin sense 5′-GTGGGGCGCCCCAGGCACCA-3′antisense 5′-CTTCCTTAATGTCACGCACGATTTC-3′ 55 45