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. 2011 Oct 18;6(10):e26298. doi: 10.1371/journal.pone.0026298

Figure 4. Ectopic PDX-1 expression activated the insulin promoter in human keratinocytes in-vitro.

Figure 4

Human keratinocytes isolated from neonatal foreskin were co-infected with either the control virus Ad-β-gal or Ad-PDX-1 (both at 1000 moi) and a reporter gene of either (a–d) Ad-RIP-GFP (1000 moi,) or (e) Ad-RIP-Luciferase (100 moi,), for 7 days. (a, c) Representative phase contrast micrographs show cell morphology. (b, d) Green fluorescent protein imaging of the same fields (a&b; c&d) presented in the phase contrast. (b) control cells did not express GFP; and (d) PDX-1 stimulated GFP expression in numerous cells. (e) Luciferase activity of the infected keratinocytes. The mean ±standard deviation is expressed in relative luciferase units; data represents n≥8 samples in three independent experiments.