Skip to main content
. 2011 Oct 18;6(10):e26433. doi: 10.1371/journal.pone.0026433

Figure 4. Overexpressing SH2B3 inhibits FGF1-induced signaling and neuronal differentiation of PC12 cells.

Figure 4

(A) PC12, PC12-SH2B1β and PC12-SH2B3 cells were treated with 100 ng/ml of FGF1 together with 10 µg/ml of heparin for 6 days. Representative images of live cells are shown. Scale bar: 50 µm. (B) PC12 cells were treated as in (A), differentiation rate of PC12, PC12-SH2B1β, or PC12-SH2B3 cells on Day 6 was analyzed as described in the Materials and Methods from three independent experiments. *: P<0.05, paired Student t-test. (C) PC12-GFP, PC12-SH2B1β and PC12-SH2B3 cells were treated with 100 ng/ml of FGF1 together with 10 µg/ml of heparin for 0, 5, or 10 min. Cell lysates were collected, analyzed via western blotting using anti-pERK1/2 and anti-ERK1/2 antibodies. (D) PC12-GFP, PC12-SH2B1β and PC12-SH2B3 cells were treated with 100 ng/ml of FGF1 together with 10 µg/ml of heparin for 0, 0.5, or 1 h. Cell lysates were analyzed via western blotting using anti-Egr-1 and anti-tubulin antibodies. Tubulin levels serve as loading controls.