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. 2011 Oct 18;6(10):e26306. doi: 10.1371/journal.pone.0026306

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Fu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Image of a Daphnia in a chamber on the stage of a microscope (Nikon ECLIPSE Ti) taken with a commercial camera (Nikon D90). The beating heart is clearly observed (see supplementary material Movie S1). B) A differential image (obtained by subtracting adjacent frames) of the heart in full-frame mode (see supplementary material Movie S2). Image were acquired at 24.6 fps and captured simultaneously with a high-speed ROI. C) ROI acquired at high-speed (1572 fps). The size of the ROI is 512 pixels (see supplementary material Movie S3).