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. 2011 Oct 18;6(10):e26391. doi: 10.1371/journal.pone.0026391

Figure 5. Resveratrol improves cardiac and soleus muscle fatty acid utilization.

Figure 5

(A) Mitochondrial substrate utilization assessed by respiration of permeabilized cardiac fibers using cumulative addition of 2 mM ADP 4 mM malate, 1 mM carnitine and 0.1 mM palmitoylCoA, 0.15 mM palmitoylcarnitine, 1 mM pyruvate and 10 mM glutamate. (B) Respiration normalized to the maximal respiration rate and measured in the presence of all of the substrates revealed decreased fatty acid oxidation. (C) mRNA levels of PPARα (peroxisome proliferator-activated receptor α), LCAD (long-chain acylCoA dehydrogenase), MCAD (middle-chain acylCoA dehydrogenase), CPT-Ib (carnitine palmitoyltransferase Ib), PDK4 (pyruvate dehydrogenase kinase 4). (D) Mitochondrial substrate utilization of permeabilized soleus fibers. (E) Normalized respiration, indicating decreased fatty acid oxidation by soleus mitochondria. Between 7 and 9 animals per group. mRNA levels were normalized to LS group taken as 1. *P<0.05, **P<0.01, ***P<0.001 vs LS; $P<0.05, $$P<0.01, $$$P<0.001 vs HS-NT.