Figure 4. Refolding assays of bovine rhodanese performed under non-permissive conditions at 25°C.

A. Refolding profiles of rhodanese in the presence of GroES, ATP and wild type/CP GroEL. Open circles indicate spontaneous refolding, closed circles indicate refolding in the presence of wild type GroEL. The other traces were measured in the presence of the following CP mutants: closed triangles, CP209, open triangles, CP254, open squares, CP376. B. The refolding of rhodanese was a GroES-dependent event. Refolding yields after a 60 min incubation at 25°C are shown. The grey column indicates spontaneous yields, and for each chaperonin, the solid column denotes refolding yields in the absence of GroES and the hatched column indicates yield in the presence of equimolar GroES.