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. 2011 Oct 18;6(10):e26462. doi: 10.1371/journal.pone.0026462

Figure 8. Proteinase K digestion of the BeFx-induced football complexes of GroEL R231W and GroEL CP376.

Figure 8

Arrested football complexes of GroEL R231W and GroEL CP376-RW were prepared and incubated at 25°C. The figure shows a 15% SDS-PAGE gel of samples concentrated and recovered after a 30 min digestion with 1 µg/ml Proteinase K at 25°C. The apparent molecular weights of a commercial marker mixture (Dalton VII molecular weight marker, Sigma) are indicated to the left of the figure. Rho denotes unfolded rhodanese, with plus and minus tags indicating whether this unfolded protein was allowed to bind to each chaperonin prior to construction of the football complex. The arrowhead indicates the position of recovered rhodanese.