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. 2011 Oct 18;6(10):e26454. doi: 10.1371/journal.pone.0026454

Figure 1. Biochemical identification of PGRN-interacting proteins.

Figure 1

(A) mPGRN-AP or mPGRN-HA fusion protein constructs were stably transfected into HEK293 cells. Cell lysates or culture medium was analyzed on Western blot with anti-mPGRN antibody. This antibody specifically recognizes mPGRN but not the endogenous hPGRN in HEK293 cells. β-actin was used as the loading control. (B) Stably transfected HEK293 cells expressing mPGRN-HA were treated with cross linkers, followed by immunoprecipitation (IP) with HA antibody or control IgG. Immunoisolates were analyzed on Western blot with anti-mPGRN antibody. UT, untransfected HEK293 cells; ST, stably transfected cells.