Skip to main content
. 2011 Oct 18;6(10):e26454. doi: 10.1371/journal.pone.0026454

Figure 6. Loss of ERp57 activity caused reduced PGRN secretion.

Figure 6

(A) Western blot analysis of the efficiencies of siRNA knockdown of ERp57 expression in stably transfected HEK293 cells expressing mPGRN. β-tubulin was used as the loading control. (B) qRT-PCR analysis of relative mPGRN mRNA levels after siRNA treatment. (C) mPGRN levels in the culture medium were measured by Western blot with anti-mPGRN antibody. Asterisk indicates a protein band of unknown identity visualized by staining of the Western blot membrane with Ponceau S to indicate equal loading. (D) Quantification of mPGRN levels in C. The values are mean ± SEM. *p<0.02, **p<0.002 vs. scrambled control. This experiment was repeated three times with similar results. (E) A vector-based shRNA construct reduced ERp5 expression level in stably transfected HEK293 cells expressing mPGRN. β-tubulin was used as the loading control. (F) Levels of secreted mPGRN were reduced in the culture medium of HEK293 cells with partial knockdown of ERp5. Asterisk indicates a protein band of unknown identity visualized by staining of the western blot membrane to indicate equal loading. (G) Quantification of the levels of mPGRN in F. Values are mean ± SEM. *p<0.02. This experiment was repeated three times with similar results.