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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Curr Protoc Chem Biol. 2011 Apr 1;3(2):53–63. doi: 10.1002/9780470559277.ch100194

Fig. 3.

Fig. 3

Comparison of 4 different antibodies binding to open-ring and closed-ring AEAB conjugates. The arrays were printed using a piezo printer (Perkin Elmer) with open- and closed-ring AEAB derivatives at 300 μM on NHS-derivatized slides. Antibodies were applied to the glycan array at the indicated concentrations above and detected with appropriate fluorescently labeled secondary antibodies (Song et al., 2009). The X-axis represents different glycans and the Y-axis represents the relative fluorescent unit (RFU) detected on the microarray.