Figure 1.
Systemic valproate administration promotes histone hyperacetylation in the brain. Immunoflourescent images show the presence of acetylated histone 3 Lys9 (AcH3 Lys 9) in the substantia nigra (outline) of saline (A) or VPA treated (B) mice (scale bar = 100µm). Representative western blots show increased acetylation of AcH3 Lys 9 in frontal cortex in animals receiving VPA as well as VPA and MPTP, compared to control animals (C). Densitometric analysis showed significant increases in AcH3 Lys 9 (relative to the level of β-actin in each sample) in VPA and VPA/MPTP-treated animals, compared to their respective controls (D). **: p<0.01.
