Figure 1.
Effects of Natura-alpha (NTI) and Taxol on cell growth of androgen-independent prostate cancer (LNCaP-AI) with different treatment regimens. A, C, and E are Dose-effect curves, and B, D, and F are CI-effect curves. Dash line in B, D, and F (CI=1) is the divider of outcomes of two-drug combination. CI value above the dash line indicates an antagonistic effect, below the line is a synergistic effect, and on the line implies an additive effect. A regression analysis of CI values versus effects in B, D, and F were performed by Sigma plot 8, and the line direction indicates trend of a combination. Androgen-independent LNCaP-AI cells were treated simultaneously with NTI and Taxol (A and B); C and D (Combination 2, NTI → Taxol): the cells were exposed to NTI first for 3 days followed by Taxol treatment for additional 3 days; Panel E&F (Combination 3, Taxol → NTI): the cells were treated with Taxol first for 3 days followed by NTI treatment for additional 3 days.