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. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Clin Cancer Res. 2011 May 23;17(13):4414–4424. doi: 10.1158/1078-0432.CCR-11-0431

Effects of Natura-alpha on FOXM1 expression in vitro. A: endogenous expression of FOXM1 in both LNCaP and LNCaP-AI cells. B: Analysis of FOXM1 expression by RT-PCR and Western blot in LNCaP and LNCaP-AI. Lanes 1, 4: LNCaP, Lanes 2, 5: LNCaP-AI, Lanes 3, 6: Beta-actin and 18S RNA loading controls. C, D, E and F: expression of FOXM1 and cell proliferation in LNCaP and LNCaP-AI cells transfected with FOXM1 or control plasmid in response to Natura-alpha treatment; G and H: expression of FOXM1 and invasion in cells transfected with FOXM1 or control plasmid in response to Natura-alpha treatment; I: effects of Natura-alpha on the expression of cyclin B1, D1, and cyclin E in LNCaP-AD and LNCaP-AI cells.

Effects of Natura-alpha on FOXM1 expression in vitro. A: endogenous expression of FOXM1 in both LNCaP and LNCaP-AI cells. B: Analysis of FOXM1 expression by RT-PCR and Western blot in LNCaP and LNCaP-AI. Lanes 1, 4: LNCaP, Lanes 2, 5: LNCaP-AI, Lanes 3, 6: Beta-actin and 18S RNA loading controls. C, D, E and F: expression of FOXM1 and cell proliferation in LNCaP and LNCaP-AI cells transfected with FOXM1 or control plasmid in response to Natura-alpha treatment; G and H: expression of FOXM1 and invasion in cells transfected with FOXM1 or control plasmid in response to Natura-alpha treatment; I: effects of Natura-alpha on the expression of cyclin B1, D1, and cyclin E in LNCaP-AD and LNCaP-AI cells.