Skip to main content
. 2011 Aug 24;124(1):169–178. doi: 10.1093/toxsci/kfr213

FIG. 5.

FIG. 5.

P2 purinergic receptor antagonists increase apoptosis in OE primary cell culture. Primary OE cell cultures were pre-incubated with vehicle (saline) or P2 purinergic receptor antagonists PPADS (25μM) and suramin (100μM) for 30 min. Cells were then incubated with vehicle (saline), ATP (100μM), or UTP (100μM) for 2 h. (A) The cells were stained with PI and subjected to flow cytometric analysis. The levels of apoptosis were normalized to vehicle (n = 3–9). *p < 0.01 versus vehicle (one-way ANOVA followed by Bonferroni's post hoc test). (B) PI exclusion assay was performed. Apoptotic cells stained positive for PI (red) and nuclei were counterstained with DAPI (blue). Scale bar = 25 μm. (C) Co-localization of PI and DAPI staining was quantified and normalized to vehicle (n = 3). *p < 0.05 versus vehicle (one-way ANOVA followed by Bonferroni's post hoc test).