TABLE 6.
GSH (nmoles GSH/mg Liver Wet Weight), GSSG (nmoles GSSG/mg Liver Wet Weight), and GSSG/GSH Ratio under Each Experiment Condition and Significance of Various Comparisons
| Treatment | Dietary Se level |
||||
| Low (0.02 ppm) | Adequate (0.2 ppm) | Supplemental (2 ppm) | 2-Way ANOVA | ||
| GSH (nmoles GSH/mg liver wet weight) | Corn oil | 8.15 ± 0.93 | 6.86 ± 0.83 | 7.00 ± 0.82 | n/a |
| 1 μmol/kg PCB 126 | 8.57 ± 1.18 | 6.71 ± 0.53 | 7.12 ± 1.93 | — | |
| 5 μmol/kg PCB 126 | 8.45 ± 0.67 | 7.26 ± 0.93 | 7.60 ± 0.87 | — | |
| 2-Way ANOVA | — | n/a | — | ||
| GSSG (nmoles GSSG/mg liver wet weight) | Corn oil | 0.052 ± 0.016 | 0.062 ± 0.005 | 0.060 ± 0.009) | n/a |
| 1 μmol/kg PCB 126 | 0.042 ± 0.004 | 0.058 ± 0.010 | 0.055 ± 0.009 | — | |
| 5 μmol/kg PCB 126 | 0.043 ± 0.006 | 0.057 ± 0.007 | 0.051 ± 0.015 | — | |
| 2-Way ANOVA | # | n/a | — | ||
| GSSG/GSH | Corn oil | 0.638 ± 0.123 | 0.902 ± 0.166 | 0.853 ± 0.061 | n/a |
| Ratio (%) | 1 μmol/kg PCB 126 | 0.494 ± 0.112 | 0.863 ± 0.087 | 0.770 ± 0.183 | — |
| 5 μmol/kg PCB 126 | 0.511 ± 0.060 | 0.780 ± 0.184 | 0.667 ± 0.019 | — | |
| 2-Way ANOVA | # | n/a | — | ||
Note. Results are expressed as mean ± SEM. Each group contained four to six animals. One-way ANOVA was used to examine statistically significant differences (p < 0.05) between each PCB 126 level and the corn oil treatment (indicated by “*” if significant) and between low or supplemental and the adequate dietary Se level (indicated by “#” if significant). Significance for each factor based on two-way ANOVA is indicated in the “2-way ANOVA” rows by “#” for Se level and the “2-way ANOVA” column by “*” for PCB 126 level. All p values have been adjusted for multiple testing using Dunnett’s test. n/a, not applicable.