Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Mol Microbiol. 2011 Jul 18;81(4):1109–1124. doi: 10.1111/j.1365-2958.2011.07757.x

Fig. 4.

Fig. 4

Expression of AdoMetDC-aminopropyltransferase fusion genes in E. coli. A, HPLC profile of Accu-Tag labelled polyamines detected by fluorescence in cellular extracts of E. coli BL21 expressing AdoMetDC-aminopropyltransferase fusion genes from Chloroherpeton thalassium ATCC 35110 (red), Delftia acidovorans SPH-1 (blue), Nocardia farcinica IFM 10152 (green), Syntrophus aciditrophicus SB (orange) and empty expression plasmid (black). DAP, diaminopropane; Put, putrescine; X, unknown; Cad, cadaverine; R, fluorescent label; NSpd, norspermidine; Spd, spermidine; IS, internal standard (diaminoheptane). B, T7 affinity tag purified proteins (4 μg per lane). The bands indicated by red asterisks are the putative processed N-terminal β-subunits of the fusion proteins and the calculated molecular masses are shown. 1, C. thalassium ATCC 35110 (predicted unprocessed fusion protein 49.9 kDa, processed α-subunit 43.0 kDa, β-subunit 7.0 kDa); 2, D. acidovorans SPH-1 (estimated unprocessed fusion protein 47.1 kDa, processed α-subunit 39.9 kDa, β-subunit 7.2 kDa); 3, N. farcinica IFM 10152 (unprocessed fusion protein 44.5 kDa, processed α-subunit 37.3 kDa, β-subunit 7.3 kDa); 4, S. aciditrophicus SB (unprocessed fusion protein 39.0 kDa, processed α-subunit 32.2 kDa, β-subunit 6.8 kDa). C, Specific AdoMetDC activities of the purified N-terminally T7-tagged fusion proteins. Cthal_fus, C. thalassium ATCC 35110; Delft_fus, D. acidovorans SPH-1; Noc_fus; N. farcinica IFM 10152.