Figure 3.

Lrig1 accumulates following E2 stimulation. (A) Hormone starved ZR75-1 (left panel) or MCF7 (right panel) cells were treated with either vehicle control (VC) or 10 nM E2 for either 8 or 24 hours as indicated. Cell lysates were blotted for Lrig1 and actin (loading control). A representative blot is shown. (B) Schematic of the 4 Lrig1 enhancer elements (referred to as enh 1–4 in text) with approximate reference to the furthest downstream transcription start site (TSS) (sequence based on genome version hg18). Table lists size of ERα DNA binding region and ERE and Forkhead motif counts.