Figure 4.

The minimal 5' regulatory region of the TRY gene including the putative analyzed MYB and MYC binding sites and their substitutions. A) Schematic overview showing the relative position of the minimal promoter with respect to the start codon. Each fragment is fused to the CaMV 35S minimal promoter and to the GUS coding region followed by the nopaline synthase terminator. The white boxes symbolize the relative position of the analyzed MYB and MYC binding sites. The black crosses shows which binding site is mutated in the respective fragment. B) Summary of the GUS expression data. We distinguish between the ubiquitous expression called "basal expression" and expression in trichomes. We found two categories, basal and trichome expression and expression only in trichomes. The percentage of analyzed independent T2 lines showing the respective expression category is provided. C) List of the analyzed binding sites with their corresponding wild type nucleotide sequence and the sequence used for base substitution.