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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Biochem Parasitol. 2011 Aug 11;180(2):69–75. doi: 10.1016/j.molbiopara.2011.08.002

Figure 3. PfPrex’s primase domain binds zinc.

Figure 3

A 5μM solution of PfPrex ZBD+TOPRIM was unfolded in 50mM HEPES pH 7.5, 3M GuHCl and the amount of zinc ion released was observed by the change in A500 in the presence of 100μM PAR. Values were normalized against a serial dilution of 1–8μM zinc ions, which fell within the linear range of detection (R2= 0.9817). The asterisk (A500 = 0.143+/−0.007, 3.46μM) represents the average signal observed over three replicates.