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Role of PKC and EGF receptor in neurotensin-induced signalling in HT29 and Panc-1 cells. Panc-1(A) and HT29 (B) cells were pretreated with vehicle (0.9% NaCl or 0.5% DMSO), 3.5 μM GF109203X, or 1 μM gefitinib for 30 min before stimulation with 1 μM neurotensin (NT) for 5 min. Cells were harvested for subsequent Western analysis as described in Methods. Results represent one typical of four independent experiments.
