Figure 6.

Role of EGFR in neurotensin-induced phosphorylation of ERK, Akt, and Shc in HCT116 cells. A, Effect of EGFR inhibition on neurotensin (NT)-induced signalling. Quiescent cells were pretreated with 25 μg/ml cetuximab for 30 min before stimulation with 1 μM NT or 10 nM EGF for five min. Cells were harvested for subsequent Western analysis as described in Methods. Results represent one typical of four independent experiments. B, Effect of protease inhibition on NT-induced phosphorylation of downstream proteins. Quiescent cells were pretreated with 10 μM GM6001 for 30 min prior to stimulation with 1 μM NT or 10 nM EGF for five min. Results represent one typical of six independent experiments.